《植物生理学报》 2014, 50(6): 797-800

过表达ZmSKIP基因提高烟草抗低温胁迫的能力

叶晓霞¹, 燕芳², 龙海桂¹, 王小敏^1,*

¹玉林师范学院生命科学与技术学院, 广西玉林537000; ²重庆大学生命科学学院, 重庆400044

通信作者：王小敏；E-mail: wxmin99@163.com；Tel: 0775-2679937

摘　要：

以野生型和过表达ZmSKIP基因烟草为试材, 研究了低温胁迫下过表达ZmSKIP对烟草抗氧化能力的影响。测定了不同低温处理时间下过表达ZmSKIP转基因烟草T3代植株和野生型植株抗氧化酶如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性和丙二醛(MDA)含量以及相对电导率, 结果表明, 低温下, 相对于野生型植株, 转基因烟草具有较高的抗氧化酶活性和较低的相对电导率和MDA含量, 说明过表达ZmSKIP提高了转基因植株的耐低温胁迫能力。

关键词：ZmSKIP; 低温胁迫; 抗氧化酶; 电导率

收稿：2014-02-10   修定：2014-05-04

资助：国家自然科学基金(31360485)、广西自然科学基金(2012GXNSFBA053076)和玉林师范学院博士启动基金(G20130013)。

Overexpression of ZmSKIP Enhanced Tolerance to Chilling Stress in Tobacco (Nicotiana tabacum)

YE Xiao-Xia¹, YAN Fang², LONG Hai-Gui¹, WANG Xiao-Min^1,*

¹College of Life Science and Technology, Yulin Normal University, Yulin, Guangxi 537000, China; ²College of Life Sciences, Chongqing University, Chongqing 400044, China

Corresponding author: WANG Xiao-Min; E-mail: wxmin99@163.com; Tel: 0775-2679937

Abstract:

The wild type (WT) and transgenic tobacco (Nicotiana tabacum) lines with overexpression of ZmSKIP were used to investigate the antioxidant ability under chilling stress. The activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), malondialdehyde (MDA) content and relative electrical conductivity of T3 transgenic tobacco plants overexpressing ZmSKIP and WT tobacco plants were examined under chilling stress. Compared to WT plants, transgenic tobacco plants overexpressing ZmSKIP displayed higher antioxidant enzyme activity, lower MDA content and relative electrical conductivity under chilling stress. These results indicated that overexpression of ZmSKIP improved the plant antioxidant ability and the chilling tolerance.

Key words: ZmSKIP; chilling stress; antioxidant enzyme; electrical conductivity

全文下载PDF版本

此摘要已有　2536　人浏览